ESHRE Campus 2010
Valencia, Spain
8-12 November 2010
Organised by the ESHRE Special Interest Group “Stem Cells” in collaboration with the Instituto de Salud Carlos III, Spanish Stem Cell Bank (Centre de Medicina Regenerativa de Barcelona, Centro de Investigacion Principe Felipe – Valencia and Centro de Investigaciones Biomédicas – Granada)
For more information and registration, please click here.
Please find here some pictures of the previous hands-on course which was held in Barcelona in February 2010.
8 November 2010
08.30 – 09.00
Registration
9.00 – 09.15
Welcome address
Chairmen: Alessandra Giorgetti (Italy) & Diana Valbuena (Spain)
09.15 – 09.45
hESC derivation
Anna Veiga (Spain)
09.45 – 10.15
New hESC derivation strategies maintaining embryo viability
Carlos Simon (Spain)
10.15 – 10.45
Culture of pluripotent stem cells in defined conditions, xenobiotic free and GMP
Johan Hyllner (Sweden)
10.45 – 11.15
Coffee break
Chairmen: Rita Vassena & Maria Eugenia Poo Llanillo (Spain)
11.15 – 11.45
Freezing and thawing of pluripotent stem cells
Diana Valbuena (Spain)
11.45 – 12.15
In vivo/in vitro differentiation
Benjamin Reubinoff (Israel)
12.15 – 12.45
Spermatogonial and ovarian stem cells
Rita Vassena (Spain)
12.45 – 13.30
General Discussion
13.30 – 15.00
Lunch break
Chairmen: Angel Raya & Carlos Simón (Spain)
15.00 – 15.30
Cell reprogramming (induced pluripotent stem cells, iPS)
Angel Raya (Spain)
15.30 – 16.00
Genetic stability of pluripotent stem cells
Pablo Menéndez (Spain)
16.00 – 16.30
Large scale culture of hESC and MSC
Mahendra Rao (USA)
16.30 - 17.00
17.00 - 17.30
Stem cell replacement therapy in diabetes
Henrik Semb (Sweden)
Chairmen: Begoña Aran & Diana Valbuena (Spain)
17.30 – 18.00
Registries and stem cell banks
Glyn Stacey (United Kingdom)
18.00 – 18.30
18.30 – 19.00
Ethical aspects of pluripotent stem cell research
Guido Pennings (Belgium)
Discussion and conclusions
9 November 2010
Welcome address and hand over of material
09.00 – 10.00
Methodology for embryo culture and feeder cell preparation
10.00 – 13.00
Hands on
Media preparation
Culture and manipulation of mouse embryos
Blastomere biopsy
Use of feeder cells: human fibroblasts (HFF-1), trypsinization, irradiation, plating
Freezing and thawing
Use of extracellular matrices
Use in defined conditions
GMP conditions
13.00 – 14.00
Lunch
14.00 – 15.30
Methodology for hESC derivation
15.30 – 19.00
Derivation of mouse embryonic stem cells (mESC)
Plating of whole embryos
ICM isolation: immunosurgery and laser dissection
Observation of mESC and hESC colonies
10 November 2010
Methodology for freezing and thawing of hESC
Mechanical expansion
Enzymatic Expansion
Bulk culture
Single cell expansion
Freezing/thawing and vitrification /warming of hESC
14.30 – 15.30
Methodology for characterization
Characterization (1)
11 November 2010
Methodology for in vivo/in vitro differentiation
Embryoid body formation differentiation
Stem cell injection to the immunocompromised animals, teratoma formation and analysis
Methodology for cell reprogramming (induced pluripotent stem cells, iPS)
Hands on reprogramming 1
12 November 2010
Hands on reprogramming 2
12.00 – 13.00
Summary and final conclusions
On-line Registration form
Programme announcement