Page 128 - Eshre 2018
P. 128

  >
 Part II: Materials and Methods
  Overview of the design strategy
  S
S
e
G
lA
G
e
r
e
e
r
N
N
o
r
t
r
m
to
o
r
o
l
l
i
     Testicular tissues
Total RNA isolation
RNA Assessment
miRNA microarray
Real Time PCR
Data analysis
Mixed Atrophy
Objective: To investigate the expression profiles of microRNA (miRNA) in human testes showing different histopathological patterns.
 mC
i
-
-
C
C
C
e
el
e
e
l
l
l
A
r
l
l
l
-
-
O
O
n
r
r
r
e
n
l
es
l
y
y
st
t
   r
m
ma
al
lt
ti
i
s
s
s
su
u
e
e
                      01.07.2018
Prof. Dr. Eckart Meese Seite
 15
     >
Part II: Results
    131 miRNAs Up -regulated
66 miRNAs Down-regulated
Sertoli Cell Only (SCO)
Mixed Atrophy (MA)
35 miRNAs Up-Regulated
33 miRNAs Down-Regulated
  Germ Cell Arrest (GA)
27 miRNAs 19 miRNAs Up -regulated Down-Regulated
   01.07.2018
Prof. Dr. Eckart Meese Seite
 16
    128
Page 122 of 130
 
   126   127   128   129   130