Page 88 - Eshre 2018
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What can we use CRISPR/Cas9 for?
Disrupt genes that are working (NHEJ) Model diseases in vitro
Create models in animals
Study gene function in vitro and in vivo
Fix genes that are not working (HDR) Gene therapies for LOF diseases Transgenic animals and plants Germline editing
Guide RNA (finds the site) Cas9 (scissors)
Guide RNA (finds the site) Cas9 (scissors)
Template DNA (example to fix)
How to target the male germ line?
Probably very difficult to target spermatozoa
SSC an alternative
We need better ex‐vivo SSC culture systems Re‐introduction?
In vitro meiosis?
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