Page 39 - PCC03

Biomarkers from blood
Whole blood
unclotted blood
Plasma
White blood cells & platelets
Red blood cells
clotted blood
Serum
blood clot
Analytes depend on the source (cellular/non-cellular): DNA, RNA, Proteins, Metabolites
Non-invasive biomarkers | Prof. Dr. Martin Götte
Blood as a biofluid for endometriosis diagnostics
Rahmioglu N et al. Fertil Steril. 2014 Nov;102(5):1233-43.
+ large volumes can be collected from patients and healthy volunteers + a broad range of biomolecules can be analysed
- the complex mix of biomolecules reflects many biological processes, some of which may not be relevant to the disease
–Points to consider:
[1] timing and conditions of sample collection (physiological state, circadian rhythms, fasting status etc.)
[2] use of anticoagulants and clot accelerators (EDTA, Heparin)
[3] sample stability between collection and processing
[4] processing (centrifugation conditions)
[5] long-term storage (aliquoting/freeze-thaw cycles, -20°C vs -80°C)
Non-invasive biomarkers | Prof. Dr. Martin Götte
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PRECONGRESS COURSE 03 I BARCELONA, SPAIN – 1 JULY 2018 39