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Gene disruption. Human 2 PN model for development.
- The gene encoding OCT4 (POU5F1) was targeted in diploid human zygotes and found that blastocyst development was compromised
- Transcriptomics analysis revealed that, in POU5F1-null cells, gene expression was downregulated not only for extra-embryonic trophectoderm genes, such as CDX2, but also for regulators of the pluripotent epiblast, including NANOG.
- CRISPR–Cas9-mediated genome editing is a powerful method for investigating
gene function in the context of human development
Ruzo and Bivanlou, 2017
Genome editing reveals a role for OCT4 in human embryogenesis Nature, Fogarthy et al ,2017 PCC Genetics - ESHRE BCN 2018
Genome engenineering through CRISP/Cas 9 technology in the human germline and pluripotent stem cells. Vassena,2016
 Review of critical technical and ethical issues that should deter from employing CRISPR/Cas9 based technologies in human reproduction until clarified.
PCC Genetics - ESHRE BCN 2018
Highly efficient NHEJ mechanism to ablate the OCT4 gene in 90% of the injected embryos and demonstrated that OCT4 has different functions in human embryogenesis compared with other animal models.
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