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 Clinical application of sperm cryopreservation
 Couples undergoing infertility treatment  Cancer patients
 Surgical sterilization such as vasectomy  Pelvic or testicular surgeries
 Degenerative illnesses such as diabetes or multiple sclerosis, spinal cord disease or injury  Screening and quarantine of donor samples
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   History of sperm cryopreservation
Sperm cryopreservation has the longest history
• 1766 : Spallanzani first cryopreservation in snow and the capture of motile sperm
• 1949 : Polge et al. : freezing semen using glycerol as cryoprotectant
• 1953 : Bunge et al. : first case report of human pregnancy from a frozen sperm sample
• Untill today : Hundreds of thousands of children conceived by AI/IVF/ICSI with cryopreserved sperm
• 1950-2018 : cryopreservation methods are still used today
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  PRECONGRESS COURSE 11 I BARCELONA, SPAIN – 1 JULY 2018 51
   


















































































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