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A problem of several studies, that patients with good number of zygotes (at least 4) are randomized to day 3 or day 5 transfer. So all bad cases are excluded from extended culture. The study should be carried out per started cycles.
On the other hand, several studies showed no difference in clinical pregnancy rate between Day 3 and Day 5 embryo transfer (Utsunomiya et al., 2004; Aziminekoo et al., 2015)
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