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  Risk of monozygotic twins
• It is suggested that there is increase risk of transfer of blastocyst stage (Luke et al., 2014) with a pooled odd ratio of 3.04 (95% CI 1.54‐ 6.01). The exact reason is unknown. (Chang et al 2009).
• Other studies confirmed this finding (Franasiak et al., 2015)
 Monozygotic twins
• Over a period of 9 years, all clinical pregnancies after single embryo transfer (N=6096) were retrospectively analyzed for the incidence of monozygotic twins which was 2.2% in all cases 136/6096. Blastocyst transfer was associated with an odd ration of 2.7, 95% CI 1.36‐5.34 (Mateizel et al., 2016)
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