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Carryover in PNT and MST embryos
• PNT using normally fertilised oocytes
carryover usually <2%; none>5% (Hyslop et al 2016)
• MST using activated oocytes
carryover <2% (avg 0.2%)(Yamada et al 2016)
• MST using fertilised eggs from mutation carriers carryover 25/26 <1%; 1=<2% (Kang et al 2016)
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• •
Zygote Morula Blastocyst
MST using activated oocytes (Yamada et al 2016)
8 ES cell lines: 4=0%; 4=0.2%‐1.7% and 0% by passage 6 but one went from 1.3% to 53.2% at passage 36
MST using fertilised eggs from mutation carriers (Kang 2016)
3/18 reverted 100% mutated mtDNA by passage 10
PNT using fertilised oocytes (Hyslop et al 2016) 4= low levels <7%;
1= upward drift to 52% by passage 12
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ICM/epiblast
ES cells
ES cells
Using hES cell lines to explore the fate of carried over mtDNA