Page 121 - ESHRE2019

Activation of the embryonic genome occurs in waves
Adapted from
Schultz and Harrison, 2019
De novo gene expression after fertilization is essential for blastocyst formation
Protein targeted by knock down
Phenotype
Outcome
Morula
• E‐cadherin
Loss of compaction
Lethal
• Epithin
Loss of compaction
Lethal
• ‐catenin
Loss of compaction
Lethal
Trophectoderm
• Eomes
Incomplete trophectoderm formation
Lethal
• Cdx2
Incomplete trophectoderm formation
Lethal
• Gata3
Blastocyst stage arrest
Inner cell mass
• Oct4
Peri‐implantation arrest
Lethal
• Sox2
Absence of epiblast
Lethal
Coticchio et al., 2019
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PRECONGRESS COURSE 02 I VIENNA, AUSTRIA – 23 JUNE 2019 119