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 Both female and male genomes are required to produce the diploid genome of the new embryo
o Constructed diploid mouse embryos with two female PN (biparental gynogenones) or two male PN (biparental androgenones).
o Diploid genome derived from only one parental sex incapable of supporting complete embryogenesis.
 The maternal and paternal contributions to the embryonic genome in mammals are not equivalent
 Both genomes are required for complete embryogenesis
Ex ovo omnia
Oocyte contributions to fertilisation
 Entire cellular machinery  Organelles
 Cortical granules
 Cytoskeleton
 Transcripts and proteins  Zona pellucida
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