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Ovastacin: Oocyte-specific Astacin
Zona Pellucida
• Pioneer marker of cortical granules • Cleaves ZP2 after fertilization
J Cell Biol 197:37, 2012
Ovastacin
Sperm do not Bind to 2C Embryos if ZP2 Cleaved Crtl Mut
kD 150
100 75
50 37
C
E
E
2
2
C
C
E
E
2
2C
Zp2Mut promoter
E1E2 E3E4
OvastacinNull X
E1 Alt E
Cleavage Site Normal 165RLADENQ171
Uncleaved Cleaved
Mutant
RLGAANQ
E5 E6 E7
E18
1 kbp
A
An
n
t
ti
i
-
-
Z
ZP
P
2
2
1E2 E3 E4E5E6
E7E8
E9
Science 329:216, 2010 J Cell Biol 197:37, 2012
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PRECONGRESS COURSE 02 I VIENNA, AUSTRIA – 23 JUNE 2019 63
