Page 172 - ESHRE2019
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The Pig Model: Results
Evaluation of Decellularization Efficiency
Macroscopic assessment
Alcian Blue (GAGs): Widespread distribution, no signal at glands after DC. 20x
DNA and protein quantification
Control
F/T F
Masson’s Trichrome: removal of red (cytoplasm) and black (nuclei) signal, only blue (collagens) remain.
Control F/T F
Innovation
Foundation
17
(Campo et al., 2017)
IF of Collagen I
• Most abundant component of ECM.
40x
Control F/T
The Pig Model: Results IF of Fibronectin
• Structural support of cells.
• Evenly dispersed through ECM, unaffected by DC
protocols.
IF of Laminin
• Complex adhesion molecule.
• Around blood vessels and glandular structures.
Innovation
• •
“Master organizer” in matrix assembly. Unaffected and evenly dispersed in interstitium.
IF of Collagen IV
• Network forming basement membrane, defines interface between tissues.
• Blood vessels, myometrium and glandular structures.
Control F/T
Control F/T
(Campo et al., 2017)
Foundation
170
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