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IMPORTANT POINTS FOR HAVING MALE FUNCTIONAL GAMETES DURING GERMLINE DIFFERENTIATION FROM PLURIPOTENT STEM CELLS:
The sequence of in vivo events:
VAHSA19gene
0% methylated
Stra8
Sertoli and Leydig75c%elmlsethylated
Scp3 γH2AX
Growth factors aMndEIhOorSmIoSnes
Acrosin
100% methylated
2011
•Generation of in vitro primordial germ cell-like cells frommES and miPS through epiblast-like cells having normal global transcription profiles, epigenetic reprogramming,and cellular dynamics.
•These PGCLCs express Integrin-b3 and SSEA1 markers of spermatogonias.
•Healthy offspring with normal methylation patterns of imprinted genes.
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PRECONGRESS COURSE 13 I VIENNA, AUSTRIA – 23 JUNE 2019 35
