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Self‐renew indefinitely
EGF↑ day2 Wnt↑
P3 P9
hTS
Cell number
Ratio
hTS‐1 hTS‐2
Doubling: ~24 hr Days
hTS‐1 (♀) P11
hTS‐2 (♂) P13
Chromosome
11
Differentiation of hTS into ST
day1 day3
hTS
cAMP↑ (forskolin)
hCG
day5
Merge
ST
Fusion
Hoechst hCG
12
82
PRECONGRESS COURSE 13 I VIENNA, AUSTRIA – 23 JUNE 2019 85
