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 P-791 A novel mtDNA knockout approach to investigate mitochondrial gene mutations in mice
R. Ahmed1, H. Ma1, T. Hayama2, C. Van Dyken1, N. Marti-Gutierrez1, H. Darby1, Y. Li1, A. Koski1, E. Kang3, Y. Lee3, D. Liang1, P. Amato1, S. Mitalipov1
1Oregon Health Sciece University, Center for Embryonic Cell and Gene Therapy, Portland, U.S.A.
2Department of OB & GYN- Saiseikai South Yokohama Hospital, Center for Reproductive Medicine- Department of OB & GYN Yokohama City University Medical Center, Yokohama, Japan
3ASAN Medical Center- Olympic-ro 43-gil- Songpa-gu, ASAN Institute for Life Sciences, Seoul, Korea- South
P-792 Testicular sperm extraction derived cells conditioned medium as an in vitro niche supports germ cells development from human embryonic stem cells
F. Akyash1, R. Aflatoonian2, E. Farashahi Yazd1, J. Golzadeh1, S.S. Tahajjodi1, H. Moore3, B. Aflatoonian1
1Stem Cell Biology Research Center- Yazd Reproductive Sciences Institute- Department of Reproductive Biology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
2Department of Endocrinology and Female Infertility- Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine- ACECR, Tehran, Iran
3Centre for Stem Cell Biology CSCB- Department of Biomedical Sciences, The University of Sheffield- Western Bank- Alfred Denny Building, Sheffield S10 2TN, United Kingdom
P-793 Cumulus cells conditioned medium as an in vitro niche for differentiation of human embryonic stem cells to female germ cells
S.S. Tahajjodi1, E. Farashahi Yazd1, R. Aflatoonian2, A. Agharahimi3, F. Hajizadeh-Tafti1, F. Akyash1, H. Moore4, B. Aflatoonian1
1Stem Cell Biology Research Center- Yazd Reproductive Sciences Institute-Department of Reproductive Biology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
2Department of Endocrinology and Female Infertility - Reproductive Biomedicine Research Centre, Royan Institute for Reproductive Biomedicine, Tehran, Iran
3Research and Clinical Center for Infertility- Yazd Reproductive Sciences Institute- Department of Reproductive Biology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
4Centre for Stem Cell Biology CSCB- Department of Biomedical Sciences, The University of Sheffield- Western Bank- Alfred Denny Building, Sheffield S10 2TN, United Kingdom
P-794 Successful development of organoids from fresh and cryopreserved biopsy catheter derived endometrial tissue of subfertile women
B. Bui1, M. Boretto2, H. Kobayashi3, M. Van Hoesel4, N. Van Hoogenhuijze1, F. Broekmans1, H. Vankelecom2, H. Torrance1
1University Medical Centre Utrecht, Department of Reproductive Medicine, Utrecht, The Netherlands
2KU Leuven University of Leuven, Department of Development and Regeneration- Cluster of Stem Cell and Developmental Biology- Unit of Stem Cell Research, Leuven, Belgium
3Yamagata University, Department of Anatomy and Structural Science, Yamagata, Japan
4University Medical Centre Utrecht, Regenerative Medicine Centre Utrecht, Utrecht, The Netherlands
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