Page 73 - Eshre 2018
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Experimental models presently used to differentiate male germ cells in vitro
• Organ cultures: 1) classical/interphase method; 2) microfluidic system 3) hanging drop
• Isolated SSCs : SSC isolation, in vitro propagation and in vitro differentiation exploiting organ cultures, co-cultures with somatic testicular cells and/or xenotransplantation
• In vitro generation of germ cells from pluripotent stem cells (ESCs, iPSCs)
• Organoid cultures : reaggregation of germ cells using natural/artificial 3D testis scaffolds
Isolation of spermatogonial stem cells (SSCs) from a donor and transplantation into the testis of a busulfan treated recipient animal
rodents
SSC isolation
human (Nagano et al. 2002) SSC isolation from OA patients
Orthotopic transplantation
Xenotransplantation into immunodeficient mouse
Production of complete spermatogenesis
SSC survival up to 6 months without differentiation
Restoration/preservation of fertility
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PRECONGRESS COURSE 01 I BARCELONA, SPAIN – 1 JULY 2018 73
