Page 75 - Eshre 2018

Isolated human SSCs
In vitro propagation of human prepubertal spermatogonial stem cells ( Sadri-Ardekani et al. 2011 JAMA)
Potential clinical application in human at risk of infertility
autotransplantation
SSC isolation and in vitro propagation
In vitro generation of functional human spermatids from SSCs
2018
• Isolation of human SSCs and Sertoli cells from OA patients
• Co-culture in 3D matrigel with several factors (KSR, RA, SCF, BMP4, T)
• Spermatocytes at day 10-15
• Spermatids at day 20 capable of fertilizing mouse
oocytes. Efficiency 17,9%
Production of complete spermatogenesis
cryopreservation
Restoration/preservation of fertility
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