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In vitro derivation of mouse functional spermatids from ESCs
Mouse ESCs
induction with cytokine mix
Cell Stem Cell, 2016
PGCLCs (primordial germ cell-like cells)
co-culture with somatic testicular cells + morphogens (RA, activin A, BMPs)
Spermatocyte-like cells
co-culture with somatic testis cells + FSH, T and BPE
Spermatid-like cells
ICSI
Healthy fertile offspring
Human reprogrammed iPSCs can in vitro differentiate to meiotic and post-meiotic male germ like cells
Panula et al. 2011 Human molecular genetics
•Human iPSCs lines reprogrammed and derived from fetal and adult fibroblasts
•induction with BMPs or overexpression of intrinsic regulators (Daz, Dazl,Boule)
•only 5% differentiate to PGCLCs (primordial germ cell like cells) •Spermatocyte-like cells assessed by immunostaining •Spermatid-like cells (function was not tested)
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PRECONGRESS COURSE 01 I BARCELONA, SPAIN – 1 JULY 2018 77
