Page 78 - Eshre 2018

• Derivation of hiPSCs primed for germ cell development from fibroblast lines, using the
four reprogramming factors (OKSM) + VASA
• Characterization of primed hiPSCs, response to BMP4, analysis of premeiotic and meiotic gene expression, epigenetic status
• Xenotransplantation of OSKMV iPSCs to seminiferous tubules of busulfan treated mice
• Differentiation of PGC/ spermatogonia localized to the basal membrane of the tubules
In vitro human PGC specification from hiPSCs
Cell stem cell, 2015
•Establishment of hiPSCs Bearing Double Germline Reporters
•Differentiation of primed iPSCs into incipient mesoderm-like cells (iMeLC) •Induction of hPGC-like cells (PGCLCs) with a combination of cytokines •Characterization of epigenetic properties and surface markers of hPGCLCs
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