Page 79 - Eshre 2018
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In vitro human PGC specification from hiPSCs and hESCs
2015
• A two-step differentiation protocol to induce hiPSCs or hESCs into PGCLCs
• A combination of cytokines different from that of the Sasaki‘s study
• PGCLC characterization by gene expression analysis
Experimental models used to differentiate male germ cells in vitro
• Organ cultures: 1) classical/ interphase method; 2) microfluidic system 3) hanging drops
• Isolated SSCs : in vitro propagation, in vitro differentiation in organ cultures or co-cultures with somatic testicular cells, transplantation
• In vitro generation of germ cells from pluripotent stem cells (ESCs,iPSCs)
• Organoid cultures : reaggregation of germ cells using natural /artificial 3D testis scaffolds
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PRECONGRESS COURSE 01 I BARCELONA, SPAIN – 1 JULY 2018 79
