Page 60 - PCC08

Imprinted DNA Methylation
Erasure
Germ Cell Development Acquisition
Preimplantation Development Maintenance
Imprinted methylation disruption can lead to Imprinting Disorders: • Beckwith‐Wiedemann Syndrome
• Angelman Syndrome
• Prader‐Willi Syndrome
Denomme & Mann, Reproduction 2012
Primordial Germ Cell
Primordial Germ Cell
To investigate the impact of blastocyst aneuploidy on genomic imprinting
Warm individual donated vitrified blastocysts
Embed blastocyst in agarose bead
Bisulfite mutagenesis C>U>T
Me
C>C>C Sequencing
CpG
Black = methylated White = unmethylated
One DNA strand from One blastocyst
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Methylation
Fertilization