Page 105 - PCC11
P. 105
Summary
1. mtDNAismaternallyinherited
2. PGDisanoptionforwomenwithmtDNAdiseaseifembryos are created with a low mutation load
3. PNTisanoptionforwomenthatproduceembryoswithahigh mutation load
4. Therearetwomajortechnicalchallenges
1. Removalofthepronucleiwhilstminimisingcarryover 2. Fusionofthekaryoplasts/cytoplast
Bibliography
- Hyslop LA, Blakeley P, et al (2016) Towards clinical application of pronuclear transfer to prevent mitochondrial DNA disease. Nature 16;534(7607):383-6
- Hyslop L & Herbert M (2016) Pronuclear transfer in normally fertilised human embryos. Protocol Exchange. https://www.nature.com/protocolexchange/protocols/4807
- Human Fertilisation and Embryology Authority Code of practice (8th edition)
Page 99 of 155
PRECONGRESS COURSE 11 I BARCELONA, SPAIN – 1 JULY 2018 105
