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MtDNA carryover in PNT blastocysts
Carryover reduced if the cytoplast originates from fresh rather than vitrified oocytes
Hyslop et al 2016
Human PNT: The technical challenges
Step 2 – Fusion of karyoplasts with cytoplast
Fusogen: Inactivated viral envelope protein (HVJ-E) >90% survival & fusion
Embryo handling pipette
- large pipette to reduce risk of karyoplasts being expelled
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PRECONGRESS COURSE 11 I BARCELONA, SPAIN – 1 JULY 2018 103
