Page 101 - PCC11
P. 101
Isolator-based workstation with controlled
- Temperature - CO2
- O2
Technical challenges of PNT
Control of environmental conditions during manipulations
Step 1 – Removal of pronuclei and minimising carryover
Timing
- Survival improved if performed shortly after appearance rather than just prior to syngamy
Pipette size
- big enough to remove the PN without damaging them
- small enough to minimise the amount of surrounding cytoplasm
Chemical inhibitors used to depolymerize the actin and microtubule networks
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PRECONGRESS COURSE 11 I BARCELONA, SPAIN – 1 JULY 2018 101
