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AOA protocol @ Ghent University Hospital combines 2 activation stimuli
Mechanical + Chemical stimulus
1. CaCl2 injection during ICSI (0.1 mol/l)
2. Twofold 10’ ionophore exposure (10 M ionomycin)
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Vanden Meerschaut et al., 2014
Single or oscillatory Ca2+ signalling for AOA?
Ferrer‐Buitrago et al., 2017
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