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However, it is impossible to completely extrapolate this to spermatozoa
• Due to low tolerance of spermatozoa to permeable agents
Osmotic tolerance of spermatozoa from rat, boar, and mouse. Redrawn from Guthrie et al., 2002
• Exposure to a high concentration of permeable agents can lead to toxic and osmotic shock
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A new breakthrough came in 2002 : vitrification of human sperm without any conventional CPA
VOETTEKST / 16
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