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The same concentration of cryoprotectant, as for conventional freezing had highly toxic and osmotic effects after vitrification.
Vitrification has shown the best results with swim-up prepared spermatozoa without cryoprotectant
VOETTEKST / 17
 Conclusion
The feasibility of the cryoprotectant-free cryopreservation of human spermatozoa and DNA integrity is comparable with that of fresh sperm
VOETTEKST / 18
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