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Conclusions
 No statistically significant differences were observed in the total motility between cryoprotectant-free vitrification compared with conventional cryopreservation
 No cryoprotectants are needed, thus avoiding the lethal effects of cryoprotectant toxicity and osmotic damage specifically to spermatozoa
 Higher percentages of DNA-fragmented spermatozoa after conventional cryopreservation in comparison with cryoprotectant-free vitrification
 Simpler, faster and more cost-effective alternative to conventional cryopreservation
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