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 Cohen et al., 1997 : cryopreservation of individual sperm in isolated zonae pellucida
 Many publications with various types of containers
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What about bad quality spermatozoa ?
 Cryopreservation has not provided complete protection because motility of the preserved
spermatozoa decreases ~ 50% of their pre-freeze value
 A greater decrease is observed in spermatozoa from infertile patients compared with fertile
patients (Donnelly et al., 2001)
 Finding the spermatozoa after thawing can be labour intensive and time consuming
 For ICSI patients having motile sperm concentrated in thawed specimens would be very valuable
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PRECONGRESS COURSE 11 I BARCELONA, SPAIN – 1 JULY 2018 61