Page 60 - ESHRE2019

Sperm Binding and Fertility of Zp2Trunc Mice
Zona pellucida matrix
Zona module Zona module Zona module
2-cell
moZP1 Egg
Loss of function (necessary)
Zona pellucida matrix
moZP1
moZP2
moZP3
huZP4moZP2
Gain of function (sufficient)
moZP2Trunc
moZP3
Zona module
Zona module
2-cell
Egg
ZP2 Zona module
Role of Glycans in Sperm Binding and Fertility Zona pellucida matrix
Zona module No O-glycans (MS/MS) Zona module Zona module
moZP1
moZP2N83Q moZP3
Sperm bind
2-cell
Egg
N83QN83
Sperm bind the N-terminus of ZP2
Sperm binding to ZP2 is glycan-independent
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