Page 37 - ESHRE2019

Compensatory proliferation of diploid cells in vitro Diploid - Diploid
chimera
Diploid - Aneuploid mosaic chimera
EPI H2B-GFP mTomato
Immunosurgery
4.0 3.5 3.0 2.5 2.0 1.5 1.0 0.5 0.0
Reversine
ns
pH3 EPI H2B-GFP 80
**
Diploid
Diploid
Diploid
Aneuploid
24 hrs
Diploid
Diploid
Diploid
Aneuploid
60
40
20
0
48 hours of culture
Diploid
Diploid Aneuploid
Diploid
Diploid Diploid Diploid Aneuploid
72 hours of culture
Diploid cells compensate for the depletion of aneuploid cells from the epiblast by post-implantation elevated mitosis during implantation
Fate of aneuploid cells in mosaic mouse embryos
Diploid Aneuploid
1:1 mosaic chimera
Pre-implantation
Post-implantation
Diploid cells proliferate
Aneuploid cells in EPI undergo apoptosis
• Aneuploid cells are progressively eliminated by apoptosis from the epiblast in pre- and post-implantation embryos
• Diploid cells speed up the apoptotic elimination of aneuploid cells from the epiblast
• Diploid cells compensate for the depletion of aneuploid cells from the epiblast during implantation
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PRECONGRESS COURSE 10 I VIENNA, AUSTRIA – 23 JUNE 2019 35
Relative proportion of cells in EPI
% pH3 positive diploid cells in EPI