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Take‐home messages
We developed an efficient decellularization perfusion protocol for large reproductive organs:
• Vital proteins of the ECM are retained
• Complex composition: elastin, laminin, fibronectin, collagen type I & IV.
• Little effect of freeze‐thaw cycle • Easy storage of donor tissue.
Only slight modifications necessary to adapt to different species (rabbit): • Good possible candidate for the future decellularization of the human uterus.
DC Protocol
Innovation
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Take‐home messages
The resulting whole organ bioscaffold is biocompatible and can be converted into different categories of endometrial biomaterials.
1.
• •
2.
• •
Thin sections: Formation of organoid‐like structures
Expressing typical endometrial epithelial and stromal cell markers.
Interesting for in vitro endometrial models. Tissue‐specific hydrogels and coatings:
Usable for embryo culture and demonstrated specificity within the proliferating endometrium.
Usable both in vitro and in vivo:
• Organotypic 3D culture for investigation and drug testing.
• Treatment of pathologies such as Asherman’s syndrome or
endometrial atrophy.
Innovation
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