Page 74 - ESHRE2019
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Most abnormal hPSCs are chromosomally mosaic in culture • Studies predominantly restricted to analysis of bulk cells
• Bulk cell analysis masks low-abundance variants
• Importance of single cell analysis
http://www.allencell.org/analyses.html
Learning Objectives
Chromosomal instability in human pluripotent stem cells:
• Type and frequency of chromosomal abnormalities in hiPSCs and hESCs • Mechanisms leading to chromosomal instability hPSCs
• Culture heterogeneity
• Exogenous effects on chromosomal stability
• Implications for clinical applications
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