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Learning objectives
• Prevalence and causes of fertilization failure following ICSI
• Oocyte activation process
• Diagnostic tools in case of fertilization failure following ICSI • History of Assisted Oocyte Activation (AOA)
• Technical aspects of AOA
• Efficiency of AOA
• Safety issues
• Future perspectives
Diagnostic tools following failed fertilization through conventional ICSI
1. To diagnose the origin of the oocyte activation failure:
male (sperm borne) or female (oocyte borne)?
2. To advice on the usefulness of assisted oocyte activation (AOA) in following treatments = patient counselling.
Rybouchkin et al., 1996
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