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Diagnostic tools: 1. Mouse Oocyte Activation test (MOAT)
Allows to classify the patients based on the capcity of human sperm to activate mouse oocytes.
Method:
• Patient’s sperm is microinjected into
mouse oocytes.
• Sperm activation capacity is
represented by the % of activated oocytes at day 1.
Page 106 of 155
Rybouchkin et al., 1996
Diagnostic tools: 1. Mouse Oocyte Activation test (MOAT)
MOAT %
0 – 20% 21 – 84% 85 – 100%
MOAT group
I II III
Origin
Sperm related activation deficiency
Diminished sperm activation capacity
AOA
Normal sperm activation capacity, hence suspected oocyte‐borne activation deficiency
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