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Diagnostic tools: 2. Mouse Oocyte Calcium Analysis (MOCA)
340nm 380nm
Image every 5s
Start: 5‐10’ after ICSI Duration: 2h
Caution: MOAT and MOCA are heterologous assays
 MOAT group 3 patients with aberrant Ca2+ pattern following MOCA?
 Human PLC shows greater potency than mouse PLC to activate mouse oocytes.
Are activation rates and Ca2+ oscillatory patterns observed in mouse oocytes
fully representative of the activation capacity and Ca2+ response elicited in human oocytes? Are MOAT and MOCA sensitive enough?
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