Page 129 - PCC11

Effect of AOA on (epi)genetics
Frozen‐thawed donated MII oocytes for research.
40 min exposure to 100 M calcium‐ionophore.
Analysis: aCGH, SNP genotyping, maternal haplotyping.
Control group: embryos derived from normally fertilized oocytes.
• 79.6% activation rate (2PB1PN) (39 out of 49).
• 59.3% euploid, normal segregation of maternal chromosomes (16 out of 27).
No difference in the proportion of meiosis II type errors between artificially (28.6% [3.7% ‐ 71.0%]) and normally activated/fertilized oocytes (44.4% [13.7% ‐ 78.8%]).
Capalba et al., 2016
Learning objectives
• Prevalence and causes of fertilization failure following ICSI • Oocyte activation process
• Diagnostic tools in case of fertilization failure following ICSI • History of Assisted Oocyte Activation (AOA)
• Technical aspects of AOA • Efficiency of AOA
• Safety issues
• Future perspectives
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